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- KD/KO Validated
ATP6V0A4 Polyclonal antibody
ATP6V0A4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21570-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 21570-1-AP is ATP6V0A4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATP6V0A4 fusion protein Ag16095 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, H+ transporting, lysosomal V0 subunit a4 |
| Calculated molecular weight | 840 aa, 96 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC109305 |
| Gene symbol | ATP6V0A4 |
| Gene ID (NCBI) | 50617 |
| RRID | AB_2918072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness. This antibody was generated against the internal region of human ATP6V0A4 and is predicted to detect the a4 isoform only.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6V0A4 antibody 21570-1-AP | Download protocol |
| IHC protocol for ATP6V0A4 antibody 21570-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Lett Identification of ATP6V0A4 as a potential biomarker in renal cell carcinoma using integrated bioinformatics analysis | ||
Function (Oxf) Dmxl1 is an Essential Mammalian Gene that is Required for V-ATPase Assembly and Function In Vivo |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christopher (Verified Customer) (12-14-2020) | We used the anti-ATP6V0A4 antibody. In lysates of homozygous Atp6v0a4-KO mice (described in Hennings et al., EMBO Mol Med, 2012) the signal for ATP6V0A4 was not detected anymore. Thus, the antibody is specific and does not cross-react.
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