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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP6V1A Polyclonal antibody

ATP6V1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51152-1-AP

Synonyms

ATP6A1, ATP6V1A, ATP6V1A1, HO68, V ATPase 69 kDa subunit, V ATPase subunit A, VA68, Vacuolar ATPase isoform VA68, Vma1, VPP2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ATP6V1A Polyclonal antibody (51152-1-AP)

    WB analysis of HeLa using 51152-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ATP6V1A Polyclonal antibody (51152-1-AP)

    WB analysis of HEK-293 using 51152-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ATP6V1A Polyclonal antibody (51152-1-AP)

    WB analysis of human brain using 51152-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ATP6V1A Polyclonal antibody (51152-1-AP)

    WB analysis of mouse kidney using 51152-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 51152-1-AP (ATP6V1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP6V1A Polyclonal antibody (51152-1-AP)

    IHC staining of human testis using 51152-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ATP6V1A Polyclonal antibody (51152-1-AP)

    IHC staining of human testis using 51152-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51152-1-AP (ATP6V1A Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human brain tissue, mouse kidney tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51152-1-AP targets ATP6V1A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberNM_001690
Gene symbol ATP6V1A
Gene ID (NCBI) 523
RRIDAB_2881254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.This antibody is specific to ATP6V1A.

Protocols

Product Specific Protocols
WB protocol for ATP6V1A antibody 51152-1-APDownload protocol
IHC protocol for ATP6V1A antibody 51152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols