Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse kidney tissue, rat brain tissue, human brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
14780-1-AP targets ATP6V1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATP6V1B1 fusion protein Ag6332 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC063411 |
| Gene Symbol | ATP6V1B1 |
| Gene ID (NCBI) | 525 |
| RRID | AB_2062378 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15313 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6V1B1 antibody 14780-1-AP | Download protocol |
| IHC protocol for ATP6V1B1 antibody 14780-1-AP | Download protocol |
| IF protocol for ATP6V1B1 antibody 14780-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. | ||
Dis Model Mech Expression of N471D strumpellin leads to defects in the endolysosomal system. | ||
J Cell Physiol Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts. | ||
Environ Int Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury | ||
Mol Cell Biochem ATP6V1B1 regulates ovarian cancer progression and cisplatin sensitivity through the mTOR/autophagy pathway
| ||
Physiol Rep Expression and localization of the mechanosensitive/osmosensitive ion channel TMEM63B in the mouse urinary tract |