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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1B1 Recombinant antibody (83953-5-RR)

    WB analysis using 83953-5-RR (same clone as 83953-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using ATP6V1B1 Recombinant antibody (83953-5-RR)

    IHC staining of rat kidney using 83953-5-RR (same clone as 83953-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using ATP6V1B1 Recombinant antibody (83953-5-RR)

    IHC staining of rat kidney using 83953-5-RR (same clone as 83953-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1B1 Recombinant antibody (83953-5-RR)

    IHC staining of mouse kidney using 83953-5-RR (same clone as 83953-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1B1 Recombinant antibody (83953-5-RR)

    IHC staining of mouse kidney using 83953-5-RR (same clone as 83953-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

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ATP6V1B1 Recombinant antibody, PBS Only

ATP6V1B1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83953-5-PBS
Clone No.240955B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83953-5-PBS targets ATP6V1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP6V1B1 fusion protein Ag6332 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Calculated molecular weight 57 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC063411
Gene Symbol ATP6V1B1
Gene ID (NCBI) 525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP15313
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.