ATP6V1C1 Polyclonal antibody

Name: ATP6V1C1 Polyclonal antibody
Brand: Proteintech
SKU: 16054-1-AP

ATP6V1C1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16054-1-AP

Synonyms

ATP6C, ATP6D, ATP6V1C1, V ATPase subunit C 1, VATC, Vma5

Validation Data Gallery

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AllWBIHC

WB analysis of NIH/3T3 using 16054-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis using 16054-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 16054-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 16054-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 16054-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach cancer using 16054-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16054-1-AP (ATP6V1C1 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 16054-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, NIH/3T3 cells, human brain tissue, human testis tissue, mouse brain tissue, rat brain tissue
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 1 publications below

Product Information

16054-1-AP targets ATP6V1C1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATP6V1C1 fusion protein Ag9020 相同性解析による交差性が予測される生物種
Full Name	ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
Calculated molecular weight	382 aa, 44 kDa
Observed molecular weight	42-44 kDa
GenBank accession number	BC010960
Gene symbol	ATP6V1C1
Gene ID (NCBI)	528
RRID	AB_2062501
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ATP6V1C1 antibody 16054-1-AP	Download protocol
IHC protocol for ATP6V1C1 antibody 16054-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Ther Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells. Authors - Benfu Zhong View Article
mouse	WB	J Biol Chem A brain-enriched Drp1 isoform associates with lysosomes, late endosomes and the plasma membrane. Authors - Kie Itoh View Article
human	WB	Commun Biol A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets. Authors - Masashi Watanabe View Article
human	WB	Biochem Biophys Res Commun Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells. Authors - Jong-Jer Lee View Article
human	IHC	Bioengineered A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma. Authors - Huimin Shen View Article
human	WB	Autophagy Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate Authors - Mengting Ou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP6V1C1 Polyclonal antibody

ATP6V1C1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of NIH/3T3 using 16054-1-AP

WB analysis using 16054-1-AP

WB analysis of human brain using 16054-1-AP

WB analysis of human testis using 16054-1-AP

IHC staining of human stomach cancer using 16054-1-AP

IHC staining of human stomach cancer using 16054-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

A brain-enriched Drp1 isoform associates with lysosomes, late endosomes and the plasma membrane.

A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets.

Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.

A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma.

Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate