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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1E1 Recombinant antibody (83636-6-RR)

    WB analysis using 83636-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ATP6V1E1 Recombinant antibody (83636-6-RR)

    IHC staining of human ovary cancer using 83636-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83636-6-RR (ATP6V1E1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83636-6-RR

    Affinity and Kinetic Characterization of 83636-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83636-6-RR against Human ATP6V1E1 were performed. The affinity constant is 5.43 nM.

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ATP6V1E1 Recombinant antibody

ATP6V1E1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83636-6-RR
Clone No.240584G12

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

V-ATPase subunit E 1, V-ATPase 31 kDa subunit, P31, ATP6E2, ATP6E

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, U2OS cells, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83636-6-RR targets ATP6V1E1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP6V1E1 fusion protein Ag7399 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
Calculated molecular weight 26 kDa
Observed molecular weight31 kDa
GenBank accession numberBC004443
Gene Symbol ATP6V1E1
Gene ID (NCBI) 529
RRIDAB_3671248
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.

Protocols

Product Specific Protocols
WB protocol for ATP6V1E1 antibody 83636-6-RRDownload protocol
IHC protocol for ATP6V1E1 antibody 83636-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols