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- KD/KO Validated
ATP8A1 Polyclonal antibody
ATP8A1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 21565-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 13 publications below |
| IF | See 3 publications below |
Product Information
21565-1-AP targets ATP8A1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATP8A1 fusion protein Ag16071 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 |
| Calculated molecular weight | 1164 aa, 131 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC109317 |
| Gene symbol | ATP8A1 |
| Gene ID (NCBI) | 10396 |
| RRID | AB_10734587 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP8A1 antibody 21565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase. | ||
Proc Natl Acad Sci U S A AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies suppresses activation of YAP in alveolar epithelial type 2 cells. | ||
Sci Rep Proteomic Analysis and Functional Characterization of P4-ATPase Phospholipid Flippases from Murine Tissues. | ||
Sci Rep PPP1R12A is a recycling endosomal phosphatase that facilitates YAP activation
| ||
iScience Lipid flippase dysfunction as a therapeutic target for endosomal anomalies in Alzheimer's disease. | ||
Clin Proteomics Identification of potential molecular targets for the treatment of cluster 1 human pheochromocytoma and paraganglioma via comprehensive proteomic characterization |
