• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP8A2 Polyclonal antibody

ATP8A2 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 31697-1-AP

Synonyms

ML 1, ATPIB

Validation Data Gallery

Filter:
  • Immunoprecipitation (IP) experiment of mouse testis tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IP experiment of mouse testis using 31697-1-AP

    IP result of anti-ATP8A2 (IP:31697-1-AP, 4ug; Detection:31697-1-AP 1:600) with mouse testis tissue lysate 1120 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IHC staining of mouse brain using 31697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IHC staining of mouse brain using 31697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IHC staining of mouse testis using 31697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATP8A2 Polyclonal antibody (31697-1-AP)

    IHC staining of mouse testis using 31697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31697-1-AP (ATP8A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive IP detected inmouse testis tissue
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31697-1-AP targets ATP8A2 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP8A2 fusion protein Ag36276 相同性解析による交差性が予測される生物種
Full Name ATPase, aminophospholipid transporter-like, class I, type 8A, member 2
Calculated molecular weight133kDa
Observed molecular weight129 kDa
GenBank accession numberNM_001313741
Gene symbol ATP8A2
Gene ID (NCBI) 51761
RRIDAB_3670083
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP8A2 is also known as ATPIB. ATP8A2 is a member of the P4‐ATPase subfamily of P‐type ATPases that actively flips phosphatidylserine (PS) and phosphatidylethanolamine (PE) across membranes to generate and maintain transmembrane phospholipid asymmetry, a property important in such cellular processes as vesicle trafficking, apoptosis, cytokinesis, neuron survival, blood coagulation, and phagocytosis. ATP8A2 consists of an N‐domain involved in binding ATP, a P‐domain that undergoes phosphorylation, an A‐domain that functions in the dephosphorylation of the phosphorylated intermediate, and an M‐domain consisting of 10 transmembrane segments that comprise the pathway for translocation of the lipid across the membrane. ATP8A2 is highly expressed in the brain, spinal cord, testis, and retina (PMID: 33565221, PMID: 33766557, PMID: 37635783).

Protocols

Product Specific Protocols
IHC protocol for ATP8A2 antibody 31697-1-APDownload protocol
IP protocol for ATP8A2 antibody 31697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols