ATP9A Polyclonal antibody
ATP9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 19504-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse spleen tissue |
| Positive IHC detected in | human testis tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19504-1-AP targets ATP9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, class II, type 9A |
| Calculated molecular weight | 119 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | NM_006045 |
| Gene symbol | ATP9A |
| Gene ID (NCBI) | 10079 |
| RRID | AB_10640806 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP9A, also named as ATPIIA and KIAA0611, belongs to the cation transport ATPase (P-type) family and Type IV subfamily. ATP9A catalyzes the reaction: ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP9A antibody 19504-1-AP | Download protocol |
| IHC protocol for ATP9A antibody 19504-1-AP | Download protocol |
| IF protocol for ATP9A antibody 19504-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Joleen (Verified Customer) (06-16-2019) | It recognizes ATP9A (top most, prominent band) but also quite a few non-specific ones.
 |
