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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATPAF1 Polyclonal antibody

ATPAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15797-1-AP

Synonyms

ATP11p, ATP11 homolog, ATP11

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using ATPAF1 Polyclonal antibody (15797-1-AP)

    WB analysis of L02 using 15797-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15797-1-AP (ATPAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ATPAF1 Polyclonal antibody (15797-1-AP)

    IHC staining of human ovary cancer using 15797-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15797-1-AP (ATPAF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ATPAF1 Polyclonal antibody (15797-1-AP)

    IHC staining of human stomach cancer using 15797-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15797-1-AP (ATPAF1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman ovary cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15797-1-AP targets ATPAF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATPAF1 fusion protein Ag8506 相同性解析による交差性が予測される生物種
Full Name ATP synthase mitochondrial F1 complex assembly factor 1
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC008498
Gene symbol ATPAF1
Gene ID (NCBI) 64756
RRIDAB_2243354
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATPAF1 antibody 15797-1-APDownload protocol
IHC protocol for ATPAF1 antibody 15797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols