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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATPBD3 Polyclonal antibody

ATPBD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31537-1-AP

Synonyms

NCS6, CTU1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATPBD3 Polyclonal antibody (31537-1-AP)

    WB analysis using 31537-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 31537-1-AP (ATPBD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATPBD3 Polyclonal antibody (31537-1-AP)

    IHC staining of mouse kidney using 31537-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31537-1-AP (ATPBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using ATPBD3 Polyclonal antibody (31537-1-AP)

    IF Staining of PC-3 using 31537-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ATPBD3 antibody (31537-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31537-1-AP targets ATPBD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATPBD3 fusion protein Ag35037 相同性解析による交差性が予測される生物種
Full Name ATP binding domain 3
Calculated molecular weight36 kDa
Observed molecular weight 30-36 kDa
GenBank accession numberNM_145232
Gene symbol ATPBD3
Gene ID (NCBI) 90353
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation.

Protocols

Product Specific Protocols
WB protocol for ATPBD3 antibody 31537-1-APDownload protocol
IHC protocol for ATPBD3 antibody 31537-1-APDownload protocol
IF protocol for ATPBD3 antibody 31537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols