• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CARNS1 Polyclonal antibody

CARNS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25725-1-AP

Synonyms

ATP grasp domain containing 1, ATPGD1, Carnosine synthase 1, CARNS1, KIAA1394

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CARNS1 Polyclonal antibody (25725-1-AP)

    WB analysis using 25725-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25725-1-AP (CARNS1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CARNS1 Polyclonal antibody (25725-1-AP)

    IHC staining of mouse liver using 25725-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25725-1-AP (CARNS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CARNS1 Polyclonal antibody (25725-1-AP)

    IHC staining of mouse liver using 25725-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25725-1-AP (CARNS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

25725-1-AP targets CARNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CARNS1 fusion protein Ag22812 相同性解析による交差性が予測される生物種
Full Name ATP-grasp domain containing 1
Calculated molecular weight 827 aa, 88 kDa
Observed molecular weight 88-103 kDa
GenBank accession numberBC036557
Gene symbol CARNS1
Gene ID (NCBI) 57571
RRIDAB_2880211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CARNS1 antibody 25725-1-APDownload protocol
IHC protocol for CARNS1 antibody 25725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Transl Stroke Res

Nicotine Exposure Along with Oral Contraceptive Treatment in Female Rats Exacerbates Post-cerebral Ischemic Hypoperfusion Potentially via Altered Histamine Metabolism.

Authors - Nathan d'Adesky
View Article
ratWB

Neurochem Res

Carnosine Reduces Oxidative Stress and Reverses Attenuation of Righting and Postural Reflexes in Rats with Thioacetamide-Induced Liver Failure.

Authors - Krzysztof Milewski
View Article