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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ATRAID Polyclonal antibody (25548-1-AP)

    WB analysis of mouse brain using 25548-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse kidney tissue using ATRAID Polyclonal antibody (25548-1-AP)

    WB analysis of mouse kidney using 25548-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HEK-293 cells using ATRAID Polyclonal antibody (25548-1-AP)

    WB analysis of HEK-293 using 25548-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ATRAID Polyclonal antibody

ATRAID Polyclonal Antibody for WB, ELISA
Cat No. 25548-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Apoptosis related protein 3, APR 3, APR 3, APR3, C2orf28, HSPC013, p18, PRO240, UNQ214/PRO240

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25548-1-AP targets ATRAID in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATRAID fusion protein Ag22168 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 28
Calculated molecular weight 229 aa, 25 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberBC035850
Gene Symbol ATRAID
Gene ID (NCBI) 51374
RRIDAB_2880128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UW56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATRAID, also named as APR3, p18, C2orf28, promotes osteoblast cell differentiation and terminal mineralization. It plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway. This antibody recognizes all the three isoforms (20-24 kDa, 16-19 kDa and 28-30 kDa).

Protocols

Product Specific Protocols
WB protocol for ATRAID antibody 25548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols