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KD/KO Validated

ATX3,ATXN3 Polyclonal antibody

Name: ATX3,ATXN3 Polyclonal antibody
Brand: Proteintech
SKU: 13505-1-AP

ATX3,ATXN3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13505-1-AP

Synonyms

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

Validation Data Gallery

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AllWBIPIHC

WB analysis using 13505-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13505-1-AP
HEK-293 (WT and ATXN3 KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 13505-1-AP incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
WB analysis of HEK-293 using 13505-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 13505-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse placenta using 13505-1-AP
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 13505-1-AP
HEK-293 lysates prepared and IP of ATXN3 performed using 1.0 μg of 13505-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IHC staining of human pancreas cancer using 13505-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas cancer using 13505-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13505-1-AP (ATX3,ATXN3 antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 13505-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Neuro-2a cells, mouse heart tissue, mouse placenta tissue, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 20 publications below
IHC	See 3 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

13505-1-AP targets ATX3,ATXN3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig, caenorhabditis elegans
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATX3,ATXN3 fusion protein Ag4341 相同性解析による交差性が予測される生物種
Full Name	ataxin 3
Calculated molecular weight	370 aa, 43 kDa
Observed molecular weight	35-42 kDa
GenBank accession number	BC033711
Gene symbol	ATXN3
Gene ID (NCBI)	4287
RRID	AB_2061192
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

Protocols

Product Specific Protocols
WB protocol for ATX3,ATXN3 antibody 13505-1-AP	Download protocol
IHC protocol for ATX3,ATXN3 antibody 13505-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell An Interaction Landscape of Ubiquitin Signaling. Authors - Xiaofei Zhang View Article
human	WB	Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. Authors - Rongxuan Zhu KD Validated View Article
human	WB, IF, IP	PLoS Genet The Role of the Mammalian DNA End-processing Enzyme Polynucleotide Kinase 3'-Phosphatase in Spinocerebellar Ataxia Type 3 Pathogenesis. Authors - Arpita Chatterjee KD Validated View Article
human	WB	Sci Total Environ USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway. Authors - Lei Zhang View Article
human	WB	PLoS Genet Inactivation of PNKP by mutant ATXN3 triggers apoptosis by activating the DNA damage-response pathway in SCA3. Authors - Rui Gao View Article
mouse	WB	Neurobiol Dis A new humanized ataxin-3 knock-in mouse model combines the genetic features, pathogenesis of neurons and glia and late disease onset of SCA3/MJD. Authors - Pawel M Switonski View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATX3,ATXN3 Polyclonal antibody

ATX3,ATXN3 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 13505-1-AP

WB analysis of HEK-293 using 13505-1-AP

WB analysis of HEK-293 using 13505-1-AP

WB analysis of mouse heart using 13505-1-AP

WB analysis of mouse placenta using 13505-1-AP

IP experiment of HEK-293 using 13505-1-AP

IHC staining of human pancreas cancer using 13505-1-AP

IHC staining of human pancreas cancer using 13505-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An Interaction Landscape of Ubiquitin Signaling.

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

The Role of the Mammalian DNA End-processing Enzyme Polynucleotide Kinase 3'-Phosphatase in Spinocerebellar Ataxia Type 3 Pathogenesis.

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

Inactivation of PNKP by mutant ATXN3 triggers apoptosis by activating the DNA damage-response pathway in SCA3.

A new humanized ataxin-3 knock-in mouse model combines the genetic features, pathogenesis of neurons and glia and late disease onset of SCA3/MJD.