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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATX3,ATXN3 Monoclonal antibody

ATX3,ATXN3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F7E10

Cat no : 67057-1-Ig

Synonyms

AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of MCF-7 using 67057-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of Jurkat using 67057-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of HeLa using 67057-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of HEK-293 using 67057-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of pig brain using 67057-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    WB analysis of mouse brain using 67057-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    IHC staining of human pancreas cancer using 67057-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    IHC staining of human pancreas cancer using 67057-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67057-1-Ig (ATX3,ATXN3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATX3,ATXN3 Monoclonal antibody (67057-1-Ig)

    IF Staining of A549 using 67057-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATX3,ATXN3 antibody (67057-1-Ig, Clone: 1F7E10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, Jurkat cells, HEK-293 cells, pig brain tissue, mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67057-1-Ig targets ATX3,ATXN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATX3,ATXN3 fusion protein Ag6627 相同性解析による交差性が予測される生物種
Full Name ataxin 3
Calculated molecular weight 370 aa, 43 kDa
Observed molecular weight 41-45 kDa
GenBank accession numberBC033711
Gene symbol ATXN3
Gene ID (NCBI) 4287
RRIDAB_2882368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

Protocols

Product Specific Protocols
WB protocol for ATX3,ATXN3 antibody 67057-1-IgDownload protocol
IHC protocol for ATX3,ATXN3 antibody 67057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Clin Invest

CRISPR screening identifies the deubiquitylase ATXN3 as a PD-L1-positive regulator for tumor immune evasion

Authors - Shengnan Wang
View Article
humanWB

J Biol Chem

ATXN3 functions as a tumor suppresser through potentiating Galectin-9-mediated apoptosis in human colon adenocarcinoma

Authors - Yang Cheng
View Article