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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATXN10 Polyclonal antibody

ATXN10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15693-1-AP

Synonyms

SCA10, E46L, Ataxin-10, ataxin 10

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ATXN10 Polyclonal antibody (15693-1-AP)

    WB analysis of human brain using 15693-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using ATXN10 Polyclonal antibody (15693-1-AP)

    WB analysis of human kidney using 15693-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ATXN10 Polyclonal antibody (15693-1-AP)

    WB analysis of HEK-293 using 15693-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human cerebellum tissue using ATXN10 Polyclonal antibody (15693-1-AP)

    WB analysis of human cerebellum using 15693-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ATXN10 Polyclonal antibody (15693-1-AP)

    WB analysis of HeLa using 15693-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15693-1-AP (ATXN10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using ATXN10 Polyclonal antibody (15693-1-AP)

    IP experiment of A431 using 15693-1-AP

    IP result of anti-ATXN10 (IP:15693-1-AP, 4ug; Detection:15693-1-AP 1:1000) with A431 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATXN10 Polyclonal antibody (15693-1-AP)

    IHC staining of human ovary tumor using 15693-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATXN10 Polyclonal antibody (15693-1-AP)

    IHC staining of human ovary tumor using 15693-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15693-1-AP (ATXN10 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATXN10 Polyclonal antibody (15693-1-AP)

    IF Staining of HeLa using 15693-1-AP

    Immunofluorescent analysis of Hela cells, using ATXN10 antibody 15693-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, human kidney tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15693-1-AP targets ATXN10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATXN10 fusion protein Ag8259 相同性解析による交差性が予測される生物種
Full Name ataxin 10
Calculated molecular weight 53 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC007508
Gene symbol ATXN10
Gene ID (NCBI) 25814
RRIDAB_2061167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.

Protocols

Product Specific Protocols
WB protocol for ATXN10 antibody 15693-1-APDownload protocol
IHC protocol for ATXN10 antibody 15693-1-APDownload protocol
IF protocol for ATXN10 antibody 15693-1-APDownload protocol
IP protocol for ATXN10 antibody 15693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.

Authors - Sang Liyun L
View Article
humanWB

J Neurochem

Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.

Authors - Robert J Andrew
View Article