ATXN10 Polyclonal antibody
ATXN10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15693-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue, HEK-293 cells, HeLa cells, human cerebellum tissue, human kidney tissue |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
15693-1-AP targets ATXN10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATXN10 fusion protein Ag8259 相同性解析による交差性が予測される生物種 |
| Full Name | ataxin 10 |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC007508 |
| Gene symbol | ATXN10 |
| Gene ID (NCBI) | 25814 |
| RRID | AB_2061167 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATXN10, also named as SCA10, is a 475 amino acid protein, which belongs to the ataxin-10 family. ATXN10 is expressed in the central nervous system.ATXN10 is necessary for the survival of cerebellar neurons. ATXN10 may play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATXN10 antibody 15693-1-AP | Download protocol |
| IHC protocol for ATXN10 antibody 15693-1-AP | Download protocol |
| IF protocol for ATXN10 antibody 15693-1-AP | Download protocol |
| IP protocol for ATXN10 antibody 15693-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways. | ||
bioRxiv Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties | ||
J Neurochem Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms. |
