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  • Featured Product
  • KD/KO Validated

AURKA Polyclonal antibody

AURKA Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28371-1-AP

Synonyms

Aurora A, aurora kinase A, AURK, AURA, ARK-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AURKA Polyclonal antibody (28371-1-AP)

    WB analysis using 28371-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28371-1-AP (AURKA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AURKA Polyclonal antibody (28371-1-AP)

    WB analysis of HEK-293 using 28371-1-AP

    WB result of AURKA antibody (28371-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AURKA transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AURKA Polyclonal antibody (28371-1-AP)

    IF Staining of HeLa using 28371-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (28371-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using AURKA Polyclonal antibody (28371-1-AP)

    IF Staining of Caco-2 using 28371-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using AURKA antibody (28371-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, SW 1990 cells
Positive IF/ICC detected inHeLa cells, Caco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28371-1-AP targets AURKA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AURKA fusion protein Ag29006 相同性解析による交差性が予測される生物種
Full Name aurora kinase A
Calculated molecular weight 46 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC002499
Gene symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Protocols

Product Specific Protocols
WB protocol for AURKA antibody 28371-1-APDownload protocol
IF protocol for AURKA antibody 28371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols