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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AXIN1 Monoclonal antibody, PBS Only

AXIN1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C4E8

Cat no : 68093-1-PBS

Synonyms

hAxin, Axis inhibition protein 1, Axin-1, AXIN 1, AXIN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AXIN1 Monoclonal antibody (68093-1-Ig)

    WB analysis using 68093-1-Ig (same clone as 68093-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68093-1-Ig (AXIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using AXIN1 Monoclonal antibody (68093-1-Ig)

    WB analysis of HSC-T6 using 68093-1-Ig (same clone as 68093-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68093-1-Ig (AXIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using AXIN1 Monoclonal antibody (68093-1-Ig)

    WB analysis of NIH/3T3 using 68093-1-Ig (same clone as 68093-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68093-1-Ig (AXIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using AXIN1 Monoclonal antibody (68093-1-Ig)

    IHC staining of mouse stomach using 68093-1-Ig (same clone as 68093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using AXIN1 Monoclonal antibody (68093-1-Ig)

    IHC staining of mouse stomach using 68093-1-Ig (same clone as 68093-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68093-1-Ig (AXIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using AXIN1 Monoclonal antibody (68093-1-Ig)

    IF Staining of A431 using 68093-1-Ig (same clone as 68093-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using AXIN1 antibody (68093-1-Ig, Clone: 1C4E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using AXIN1 Monoclonal antibody (68093-1-Ig)

    FC experiment of A431 using 68093-1-Ig (same clone as 68093-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human AXIN1 (68093-1-Ig, Clone:1C4E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68093-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68093-1-PBS targets AXIN1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AXIN1 fusion protein Ag10079 相同性解析による交差性が予測される生物種
Full Name axin 1
Calculated molecular weight826aa,92 kDa; 862aa,95 kDa
Observed molecular weight110-120 kDa
GenBank accession numberBC044648
Gene symbol AXIN1
Gene ID (NCBI) 8312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Recent researches find that CircAXIN1 encodes a novel protein, AXIN1-295aa, which shows at around 40-55 kDa by Western Blot. AXIN1-295aa functions as an oncogenic protein, activating the Wnt signaling pathway to promote GC tumorigenesis and progression, suggesting a potential therapeutic target for GC.