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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AXL Recombinant antibody (84659-1-RR)

    WB analysis using 84659-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84659-1-RR (Axl antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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AXL Recombinant antibody

AXL Recombinant Antibody for WB, ELISA
Cat No. 84659-1-RR
Clone No.242014B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

UFO, Tyrosine-protein kinase receptor UFO, EC:2.7.10.1, 242014B9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, NCI-H1299 cells, A431 cells, U2OS cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84659-1-RR targets AXL in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name AXL receptor tyrosine kinase
Calculated molecular weight98kDa
Observed molecular weight130-140 kDa
GenBank accession numberNM_021913.4
Gene Symbol AXL
Gene ID (NCBI) 558
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30530-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

Protocols

Product Specific Protocols
WB protocol for AXL antibody 84659-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols