Zinc Alpha 2 Glycoprotein Polyclonal antibody
Zinc Alpha 2 Glycoprotein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 13399-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma, MCF-7 cells |
| Positive IHC detected in | mouse liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 4 publications below |
Product Information
13399-1-AP targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Zinc Alpha 2 Glycoprotein fusion protein Ag4232 相同性解析による交差性が予測される生物種 |
| Full Name | alpha-2-glycoprotein 1, zinc-binding |
| Calculated molecular weight | 298 aa, 34 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC033830 |
| Gene symbol | Zinc Alpha 2 Glycoprotein |
| Gene ID (NCBI) | 563 |
| RRID | AB_2062582 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-AP | Download protocol |
| IHC protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-AP | Download protocol |
| IF protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis. | ||
Int J Cancer Reduced AZGP1 expression is an independent predictor of early PSA recurrence and associated with ERG fusion positive and PTEN deleted prostate cancers. | ||
J Transl Med Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis. | ||
Proteome Sci Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography. | ||
