Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IF | See 5 publications below |
| IP | See 2 publications below |
Product Information
25735-1-AP targets AZI1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AZI1 fusion protein Ag22699 相同性解析による交差性が予測される生物種 |
| Full Name | 5-azacytidine induced 1 |
| Calculated molecular weight | 1083 aa, 122 kDa |
| Observed molecular weight | 131 kDa |
| GenBank accession number | BC136578 |
| Gene Symbol | AZI1 |
| Gene ID (NCBI) | 22994 |
| RRID | AB_2880216 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPN4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AZI1 antibody 25735-1-AP | Download protocol |
| IF protocol for AZI1 antibody 25735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun USP9X regulates centrosome duplication and promotes breast carcinogenesis.
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PLoS Biol Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium. | ||
Front Oncol USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.
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