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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Acetyl-Histone H2B (Lys120) Recombinant antibody

Acetyl-Histone H2B (Lys120) Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241556E7

Cat no : 84551-1-RR

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H2B (Lys120) Recombinant antibody (84551-1-RR)

    WB analysis of NIH/3T3 using 84551-1-RR

    Trichostatin A treated NIH/3T3 cells and untreated cells were subjected to SDS PAGE followed by western blot with 84551-1-RR (Acetyl-Histone H2B (Lys120) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2B (Lys120) Recombinant antibody (84551-1-RR)

    IF Staining of HeLa using 84551-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys120) antibody (84551-1-RR, Clone: 241556E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of peptide using Acetyl-Histone H2B (Lys120) Recombinant antibody (84551-1-RR)

    Dot Blot experiment of peptide using 84551-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys120) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF/ICC detected inTrichostatin A treated HeLa cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84551-1-RR targets Acetyl-Histone H2B (Lys120) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
GenBank accession numberBC005827
Gene symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys120) antibody 84551-1-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys120) antibody 84551-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols