Validation Data Gallery
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Tested Applications
| Positive WB detected in | Trichostatin A treated NIH/3T3 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive Dot Blot detected in | peptide |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84549-1-RR targets Acetyl-Histone H2B (Lys20) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 2, H2be |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC005827 |
| Gene Symbol | Histone H2B |
| Gene ID (NCBI) | 8349 |
| RRID | AB_3672056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q16778 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Acetyl-Histone H2B (Lys20) antibody 84549-1-RR | Download protocol |
| IF protocol for Acetyl-Histone H2B (Lys20) antibody 84549-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |