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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Acetyl-Histone H3 (Lys18) Recombinant antibody

Acetyl-Histone H3 (Lys18) Recombinant Antibody for WB, ELISA, Dot Blot

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA, Dot Blot

Conjugate

Unconjugated

CloneNo.

2E3

Cat no : 82832-1-RR

Synonyms

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H3 (Lys18) Recombinant antibody (82832-1-RR)

    WB analysis of NIH/3T3 using 82832-1-RR

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82832-1-RR (Acetyl-Histone H3 (Lys18) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Dot Blot experiment of peptide using Acetyl-Histone H3 (Lys18) Recombinant antibody (82832-1-RR)

    Dot Blot experiment of peptide using 82832-1-RR

    Dot blot analysis was used to confirm the specificity of 82832-1-RR Acetyl-Histone H3 (Lys18) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. Column 1: H3AK18Ac. Column 2: Unmodified H3AK18. Column 3: H3AK36Ac. Column 4: Unmodified H3AK36. Column 5: H3AK23Ac. Column 6: Unmodified H3AK23. Column 7: H3AK122Ac. Column 8:Unmodified H3AK122. Column 9: H3AK27Ac. Column 10: Unmodified H3AK27. Column 11: H3AK14Ac. Column 12: Unmodified H3AK14. Column 13: H3AK56Ac. Column 14:Unmodified H3AK56. Column 15:H3AK9Ac. Column 16:Unmodified H3AK9. Column 17:H3AK4Ac. Column 18:Unmodified H3AK4. Column 19:Blank(PBS).

Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82832-1-RR targets Acetyl-Histone H3 (Lys18) in WB, ELISA, Dot Blot applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys18) antibody 82832-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols