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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Acetyl-Histone H3 (Lys4) Recombinant antibody (84831-1-RR)

    WB analysis of HeLa using 84831-1-RR

    Trichostatin A treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 84831-1-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H3 (Lys4) Recombinant antibody (84831-1-RR)

    WB analysis of NIH/3T3 using 84831-1-RR

    Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84831-1-RR (HIST1H3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H3 (Lys4) Recombinant antibody (84831-1-RR)

    IF Staining of HeLa using 84831-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84831-1-RR, Clone: 241882E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of peptide using Acetyl-Histone H3 (Lys4) Recombinant antibody (84831-1-RR)

    Dot Blot experiment of peptide using 84831-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys4) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

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Acetyl-Histone H3 (Lys4) Recombinant antibody

Acetyl-Histone H3 (Lys4) Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA
Cat No. 84831-1-RR
Clone No.241882E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, ELISA

Synonyms

Acetyl Histone H3, Ac Histone H3-Lys4, Ac Histone H3 Lys4, Ac Histone H3 (Lys4), Ac Histone H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 647
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells, Trichostatin A treated HeLa cells
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84831-1-RR targets Acetyl-Histone H3 (Lys4) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3672140
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys4) antibody 84831-1-RRDownload protocol
IF protocol for Acetyl-Histone H3 (Lys4) antibody 84831-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols