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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Acetyl-Histone H4 (Lys12) Recombinant antibody

Acetyl-Histone H4 (Lys12) Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D18

Cat no : CL488-83095

Synonyms

HIST1H4A, 1D18, Histone-H4, histoneH4, Histone H4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CoraLite® Plus 488-conjugated Acetyl-Histone H4 (L (CL488-83095)

    IF Staining of Caco-2 using CL488-83095

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CoraLite® Plus 488 Acetyl-Histone H4 (Lys12) antibody (CL488-83095, Clone: 1D18 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83095 targets Acetyl-Histone H4 (Lys12) in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H4a
Observed molecular weight12 kDa
GenBank accession numberBC069654
Gene symbol HIST1H4A
Gene ID (NCBI) 8359
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Acetylation of histone H4 is necessary for chromatin decompaction during DNA replication.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Acetyl-Histone H4 (Lys12) antibody CL488-83095Download protocol
Standard Protocols
Click here to view our Standard Protocols