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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Adiponectin Monoclonal antibody, PBS Only

Adiponectin Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D8A7

Cat no : 66239-1-PBS

Synonyms

ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human adipose tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    WB analysis of human adipose using 66239-1-Ig (same clone as 66239-1-PBS)

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66239-1-Ig (ADIPOQ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of mouse skin using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 66239-1-Ig (ADIPOQ antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of mouse skeletal muscle using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of mouse skeletal muscle using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of mouse skin using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 66239-1-Ig (ADIPOQ antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of human placenta using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of human placenta using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of human prostate cancer using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of human prostate cancer using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of mouse brown adipose using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of mouse brown adipose using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brown adipose tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of rat brown adipose using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brown adipose tissue using Adiponectin Monoclonal antibody (66239-1-Ig)

    IHC staining of rat brown adipose using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of 3T3-L1 cells using Adiponectin Monoclonal antibody (66239-1-Ig)

    IF Staining of 3T3-L1 using 66239-1-Ig (same clone as 66239-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using Adiponectin antibody (66239-1-Ig, Clone: 5D8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66239-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66239-1-PBS targets Adiponectin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Adiponectin fusion protein Ag17383 相同性解析による交差性が予測される生物種
Full Name adiponectin, C1Q and collagen domain containing
Calculated molecular weight 244 aa, 26 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC096308
Gene symbol ADIPOQ
Gene ID (NCBI) 9370
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.