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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Adiponectin Recombinant antibody

Adiponectin Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241082C11

Cat no : 83961-1-RR

Synonyms

ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of 3T3-L1 cells using Adiponectin Recombinant antibody (83961-1-RR)

    IF Staining of 3T3-L1 using 83961-1-RR

    Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using Adiponectin antibody (83961-1-RR, Clone: 241082C11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83961-1-RR

    Affinity and Kinetic Characterization of 83961-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83961-1-RR against Human Adiponectin were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected in3T3-L1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83961-1-RR is Adiponectin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Adiponectin fusion protein Ag16304 相同性解析による交差性が予測される生物種
Full Name adiponectin, C1Q and collagen domain containing
Calculated molecular weight 244 aa, 26 kDa
GenBank accession numberBC096308
Gene symbol Adiponectin
Gene ID (NCBI) 9370
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Protocols

Product Specific Protocols
IF protocol for Adiponectin antibody 83961-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols