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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Adiponectin receptor 1 Polyclonal antibody

Adiponectin receptor 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22425-1-AP

Synonyms

ADIPOR1, Adiponectin receptor protein 1, PAQR1, CGI-45, CGI45

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Adiponectin receptor 1 Polyclonal antibody (22425-1-AP)

    WB analysis using 22425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22425-1-AP (Adiponectin receptor 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Adiponectin receptor 1 Polyclonal antibody (22425-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 22425-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22425-1-AP (Adiponectin receptor 1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Adiponectin receptor 1 Polyclonal antibody (22425-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 22425-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 22425-1-AP (Adiponectin receptor 1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22425-1-AP targets Adiponectin receptor 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Adiponectin receptor 1 fusion protein Ag18187 相同性解析による交差性が予測される生物種
Full Name adiponectin receptor 1
Calculated molecular weight 375 aa, 43 kDa
Observed molecular weight45 kDa
GenBank accession numberBC010743
Gene symbol Adiponectin receptor 1
Gene ID (NCBI) 51094
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin have been identified: adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2). They are seven-transmembrane domain receptors and mediate increased AMPK, MAPK and PPARA ligand activity in response to adiponectin. AdipoR1 is found in many tissues, being particularly abundant in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver (PMID: 24417942). AdipoR1 has a high affinity for globular adiponectin and a very low affinity for full-length adiponectin. AdipoR2 has an intermediate affinity for both forms.

Protocols

Product Specific Protocols
WB protocol for Adiponectin receptor 1 antibody 22425-1-APDownload protocol
IHC protocol for Adiponectin receptor 1 antibody 22425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols