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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Rat Adiponectin Recombinant antibody, PBS Only (Detector)

Adiponectin Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240392D6

Cat no : 83525-2-PBS

Synonyms

Adipoq, Acrp30, Acdc, 240392D6

Validation Data Gallery

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  • Western Blot (WB) analysis of rat adipose tissue using Adiponectin Recombinant antibody (83525-2-RR)

    WB analysis of rat adipose using 83525-2-RR (same clone as 83525-2-PBS)

    rat adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adiponectin antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83525-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse adipose tissue using Adiponectin Recombinant antibody (83525-2-RR)

    WB analysis of mouse adipose using 83525-2-RR (same clone as 83525-2-PBS)

    mouse adipose tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83525-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Adiponectin Recombinant antibody (83525-2-RR)

    WB analysis of rat heart using 83525-2-RR (same clone as 83525-2-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 83525-2-RR (Adipoq antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83525-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00522-1

    Cytometric bead array standard curve of MP00522-1

    Cytometric bead array standard curve of MP00522-1, RAT Adiponectin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83525-3-PBS. Detection antibody: 83525-2-PBS. Standard: Eg0872. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00522-2

    Cytometric bead array standard curve of MP00522-2

    Cytometric bead array standard curve of MP00522-2, RAT Adiponectin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83525-1-PBS. Detection antibody: 83525-2-PBS. Standard: Eg0872. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83525-2-RR

    Affinity and Kinetic Characterization of 83525-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83525-2-RR against Rat Adiponectin were performed. The affinity constant is 0.149 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83525-2-PBS targets Adiponectin as part of a matched antibody pair:

MP00522-1: 83525-3-PBS capture and 83525-2-PBS detection (validated in Cytometric bead array)

MP00522-2: 83525-1-PBS capture and 83525-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name adiponectin, C1Q and collagen domain containing
Calculated molecular weight26 kDa
Observed molecular weight26-28 kDa
GenBank accession numberNM_144744.3
Gene symbol Adiponectin
Gene ID (NCBI) 246253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.