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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Adropin Polyclonal antibody

Adropin Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25213-1-AP

Synonyms

Adropin, C9orf165, energy homeostasis associated, ENHO, UNQ470

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using Adropin Polyclonal antibody (25213-1-AP)

    IHC staining of human liver using 25213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25213-1-AP (Adropin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Adropin Polyclonal antibody (25213-1-AP)

    IHC staining of human liver using 25213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25213-1-AP (Adropin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Adropin Polyclonal antibody (25213-1-AP)

    IF Staining of SH-SY5Y using 25213-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Adropin antibody (25213-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25213-1-AP targets Adropin in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Adropin fusion protein Ag19526 相同性解析による交差性が予測される生物種
Full Name energy homeostasis associated
Calculated molecular weight 75 aa, 8 kDa
GenBank accession numberBC022101
Gene symbol Adropin
Gene ID (NCBI) 375704
RRIDAB_3085776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adropin is a nutritionally regulated peptide hormone that is primarily secreted by the liver and is important in maintaining metabolic and non-metabolic homeostasis. Adropin regulates glucose and fatty acid metabolism and exerts a potential protective effect on endothelial function through modulation of endothelial nitric oxide (NO) synthesis. It is associated with obesity and is involved in the prevention of insulin resistance, dyslipidemia, and impaired glucose tolerance (PMID: 22318315).

Protocols

Product Specific Protocols
IHC protocol for Adropin antibody 25213-1-APDownload protocol
IF protocol for Adropin antibody 25213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols