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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Afp Polyclonal antibody

Afp Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31256-1-AP

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Afp Polyclonal antibody (31256-1-AP)

    WB analysis using 31256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31256-1-AP (Afp antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Afp Polyclonal antibody (31256-1-AP)

    WB analysis using 31256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31256-1-AP (Afp antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse placenta tissue, HepG2 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31256-1-AP targets Afp in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name alpha fetoprotein
Calculated molecular weight67KD
Observed molecular weight68-72 kDa
GenBank accession numberNM_007423.4
Gene symbol Afp
Gene ID (NCBI) 11576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-fetoprotein (AFP) is one of the earliest proteins to be synthesised by the embryonic liver. The synthesis of AFP decreases dramatically after birth and only trace amounts are expressed in the adult liver. High serum AFP concentrations are also found in connection with primary liver cancer and teratocarcinoma.

Protocols

Product Specific Protocols
WB protocol for Afp antibody 31256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols