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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ajuba Polyclonal antibody

Ajuba Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28686-1-AP

Synonyms

Ajuba, JUB, Protein ajuba

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Ajuba Polyclonal antibody (28686-1-AP)

    WB analysis using 28686-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28686-1-AP (Ajuba antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of mouse skin using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of mouse skin using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of human thyroid cancer using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of human thyroid cancer using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of mouse small intestine using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of mouse small intestine using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of mouse testis using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of mouse testis using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skin tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of rat skin using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skin tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of rat skin using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of rat small intestine using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of rat small intestine using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of rat testis using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using Ajuba Polyclonal antibody (28686-1-AP)

    IHC staining of rat testis using 28686-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28686-1-AP (Ajuba antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse skin tissue, human thyroid cancer tissue, mouse small intestine tissue, mouse testis tissue, rat skin tissue, rat small intestine tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28686-1-AP targets Ajuba in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ajuba fusion protein Ag29725 相同性解析による交差性が予測される生物種
Full Name jub, ajuba homolog (Xenopus laevis)
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC007580
Gene symbol Ajuba
Gene ID (NCBI) 84962
RRIDAB_2881194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ajuba belongs to the LIM domain proteins, which are involved in the assembly of the extracellular matrix and, along with associated proteins, regulate target genes that connect the extracellular matrix and the cytoskeleton. Ajuba consists of three LIM domains at the C-terminal and is located in the cytoplasm where it interacts with a variety of other proteins. Ajuba contributes to the formation or strengthening of cell-cell adhesion and regulates cell growth and differentiation decisions. Ajuba participates in the assembly of the extracellular matrix. Ajuba also plays an important role in some diseases.

Protocols

Product Specific Protocols
WB protocol for Ajuba antibody 28686-1-APDownload protocol
IHC protocol for Ajuba antibody 28686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Authors - Yu Zhang
View Article