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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Albumin Monoclonal antibody (66051-1-Ig)

    WB analysis of human plasma using 66051-1-Ig (same clone as 66051-1-PBS)

    human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    WB analysis of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 66051-1-Ig (ALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50031-1

    Sandwich ELISA standard curve of MP50031-1

    Sandwich ELISA standard curve of MP50031-1, Albumin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66051-2-PBS. Detection antibody: 66051-1-PBS. Standard: Ag9885. Range: 50-3200 ng/mL.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Human cerebrospinal fluid (CSF) of one individual human donors was measured. The Albumin concentration of detected samples was determined to be 29.50 μg/mL.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Serum of four individual healthy human donors was measured. The Albumin concentration of detected samples was determined to be 29.65 mg/mL with a range of 11.40-48.00 mg/mL.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Urine of four individual healthy human donors was measured. The Albumin concentration of detected samples was determined to be 1,036.00 ng/mL with a range of 560.00-1,616.00 ng/mL.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Saliva of two individual healthy human donors was measured. The Albumin concentrations of detected samples were determined to be 32.00 μg/mL and 18.60 μg/mL, respectively.

  • Sample test of MP50031-1

    Sample test of MP50031-1

    Human milk of two individual healthy human donors was measured. The Albumin concentrations of detected samples were determined to be 106.00 μg/mL and 116.00 μg/mL, respectively.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IP experiment of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    IP result of anti-Albumin (IP:66051-1-Ig, 4ug; Detection:66051-1-Ig 1:1000) with HepG2 cells lysate 1720 ug. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66051-1-Ig (Albumin antibody) at dilution of 1:64000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of mouse liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of mouse liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 66051-1-Ig

    Affinity and Kinetic Characterization of 66051-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 66051-1-Ig against Human Albumin were performed. The affinity constant is 0.574 nM

Albumin Monoclonal antibody, PBS Only (Detector)

Albumin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66051-1-PBS
Clone No.4A1C11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ALB, 4A1C11, HSA, Serum albumin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66051-1-PBS targets Albumin as part of a matched antibody pair:

MP50031-1: 66051-2-PBS capture and 66051-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9885

Product name: Recombinant human Albumin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 256-609 aa of BC034023

Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

相同性解析による交差性が予測される生物種
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034023
Gene Symbol Albumin
Gene ID (NCBI) 213
RRIDAB_11042320
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP02768
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.

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