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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Albumin Monoclonal antibody (66051-1-Ig)

    WB analysis of human plasma using 66051-1-Ig (same clone as 66051-1-PBS)

    human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with 66051-1-Ig (Albumin Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    WB analysis of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 66051-1-Ig (ALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50031-1

    Sandwich ELISA standard curve of MP50031-1

    Sandwich ELISA standard curve of MP50031-1, ALB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66051-2-PBS. Detection antibody: 66051-1-PBS. Standard: Ag9885. Range: 50-3200 ng/mL.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IP experiment of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    IP result of anti-Albumin (IP:66051-1-Ig, 4ug; Detection:66051-1-Ig 1:1000) with HepG2 cells lysate 1720 ug. This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66051-1-Ig(ALB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Monoclonal antibody (66051-1-Ig)

    IHC staining of human liver using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66051-1-Ig (Albumin antibody) at dilution of 1:64000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Monoclonal antibody (66051-1-Ig)

    IF Staining of HepG2 using 66051-1-Ig (same clone as 66051-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (66051-1-Ig, Clone: 4A1C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66051-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 66051-1-Ig

    Affinity and Kinetic Characterization of 66051-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 66051-1-PBS against Human Albumin were performed. The affinity constant is 0.574 nM

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Albumin Monoclonal antibody, PBS Only (Detector)

Albumin Monoclonal Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66051-1-PBS
Clone No.4A1C11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ALB, Serum albumin, HSA, 4A1C11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66051-1-PBS targets Albumin as part of a matched antibody pair:

MP50031-1: 66051-2-PBS capture and 66051-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Albumin fusion protein Ag9885 相同性解析による交差性が予測される生物種
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034023
Gene Symbol Albumin
Gene ID (NCBI) 213
RRIDAB_11042320
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02768
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071) In additon, glycated serum albumin is also a potential diabetes biomarker.