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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Albumin Recombinant antibody

Albumin Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4K1

Cat no : 82783-6-RR

Synonyms

ALB, Serum albumin, HSA, 4K1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Albumin Recombinant antibody (82783-6-RR)

    WB analysis using 82783-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82783-6-RR (Albumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Albumin Recombinant antibody (82783-6-RR)

    IHC staining of human stomach using 82783-6-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82783-6-RR (Albumin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Albumin Recombinant antibody (82783-6-RR)

    IHC staining of human stomach using 82783-6-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82783-6-RR (Albumin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Recombinant antibody (82783-6-RR)

    IF Staining of mouse liver using 82783-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Recombinant antibody (82783-6-RR)

    IF Staining of mouse liver using 82783-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Recombinant antibody (82783-6-RR)

    IF Staining of HepG2 using 82783-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inhuman plasma, HepG2 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82783-6-RR targets Albumin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Albumin fusion protein Ag9569 相同性解析による交差性が予測される生物種
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight66 kDa
GenBank accession numberBC034023
Gene symbol Albumin
Gene ID (NCBI) 213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

Protocols

Product Specific Protocols
WB protocol for Albumin antibody 82783-6-RRDownload protocol
IHC protocol for Albumin antibody 82783-6-RRDownload protocol
IF protocol for Albumin antibody 82783-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols