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  • Featured Product
  • KD/KO Validated

Alix Monoclonal antibody, PBS Only

Alix Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9D9

Cat no : 67715-1-PBS

Synonyms

PDCD6IP, Hp95, DRIP4, ALG-2-interacting protein X, ALG-2-interacting protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alix Monoclonal antibody (67715-1-Ig)

    WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Alix Monoclonal antibody (67715-1-Ig)

    WB analysis using 67715-1-Ig (same clone as 67715-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Alix Monoclonal antibody (67715-1-Ig)

    WB analysis of HEK-293 using 67715-1-Ig (same clone as 67715-1-PBS)

    WB result of Alix antibody (67715-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alix transfected HEK-293 cells. This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Alix Monoclonal antibody (67715-1-Ig)

    IHC staining of human stomach cancer using 67715-1-Ig (same clone as 67715-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67715-1-Ig (Alix antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Alix Monoclonal antibody (67715-1-Ig)

    IHC staining of human stomach cancer using 67715-1-Ig (same clone as 67715-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67715-1-Ig (Alix antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Alix Monoclonal antibody (67715-1-Ig)

    IF Staining of HeLa using 67715-1-Ig (same clone as 67715-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Alix antibody (67715-1-Ig, Clone: 1H9D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67715-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67715-1-PBS targets Alix in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alix fusion protein Ag30437 相同性解析による交差性が予測される生物種
Full Name programmed cell death 6 interacting protein
Calculated molecular weight 868 aa, 96 kDa
Observed molecular weight100 kDa
GenBank accession numberBC020066
Gene symbol Alix
Gene ID (NCBI) 10015
RRIDAB_2882905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.