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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    WB analysis of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    WB analysis of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    WB result of Alpha 1 Antitrypsin antibody (66135-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha 1 Antitrypsin transfected HepG2 cells. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    WB analysis of rat liver using 66135-1-Ig (same clone as 66135-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    WB analysis of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    WB analysis of L02 using 66135-1-Ig (same clone as 66135-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    WB analysis using 66135-1-Ig (same clone as 66135-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human saliva using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    WB analysis of human saliva using 66135-1-Ig (same clone as 66135-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    IP experiment of human plasma using 66135-1-Ig (same clone as 66135-1-PBS)

    IP result of anti-Alpha 1 Antitrypsin (IP:66135-1-Ig, 5ug; Detection:66135-1-Ig 1:1000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha-1-Antitrypsin Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66135-1-Ig (Alpha 1 Antitrypsin antibody) at dilution of 1:64000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66135-1-Ig(Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    IHC staining of human liver using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66135-1-Ig(Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    IF Staining of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha 1 Antitrypsin antibody (66135-1-Ig, Clone: 1A9G6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Alpha-1-Antitrypsin Monoclonal antibody (66135-1-Ig)

    FC experiment of HepG2 using 66135-1-Ig (same clone as 66135-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Alpha 1 Antitrypsin (66135-1-Ig, Clone:1A9G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66135-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Alpha-1-Antitrypsin Monoclonal antibody, PBS Only

Alpha-1-Antitrypsin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 66135-1-PBS
Clone No.1A9G6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

a1-antitrypsin, Serpin A1, SERPINA1, AAT, A1AT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66135-1-PBS targets Alpha-1-Antitrypsin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha-1-Antitrypsin fusion protein Ag9516 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Calculated molecular weight 418 aa, 47 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC015642
Gene Symbol Alpha 1-Antitrypsin
Gene ID (NCBI) 5265
RRIDAB_2881534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01009
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.