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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha-1-antitrypsin Polyclonal antibody

Alpha-1-antitrypsin Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25876-1-AP

Synonyms

a1-antitrypsin, Serpin A1, SERPINA1, Alpha 1 antiproteinase, AAT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha-1-antitrypsin Polyclonal antibody (25876-1-AP)

    WB analysis using 25876-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25876-1-AP (Alpha-1-antitrypsin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using Alpha-1-antitrypsin Polyclonal antibody (25876-1-AP)

    IP experiment of human plasma using 25876-1-AP

    IP result of anti-Alpha-1-antitrypsin (IP:25876-1-AP, 4ug; Detection:25876-1-AP 1:3000) with human plasma lysate 1600 ug.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using Alpha-1-antitrypsin Polyclonal antibody (25876-1-AP)

    IHC staining of mouse placenta using 25876-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 25876-1-AP (Alpha-1-antitrypsin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman saliva, human urine
Positive IP detected inhuman plasma
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25876-1-AP targets Alpha-1-antitrypsin in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha-1-antitrypsin fusion protein Ag22631 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Observed molecular weight 47-50 kDa
GenBank accession numberBC011991
Gene symbol Alpha 1-Antitrypsin
Gene ID (NCBI) 5265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

Protocols

Product Specific Protocols
WB protocol for Alpha-1-antitrypsin antibody 25876-1-APDownload protocol
IHC protocol for Alpha-1-antitrypsin antibody 25876-1-APDownload protocol
IP protocol for Alpha-1-antitrypsin antibody 25876-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols