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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of HepG2 using 67308-1-Ig (same clone as 67308-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of HuH-7 using 67308-1-Ig (same clone as 67308-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of human plasma using 67308-1-Ig (same clone as 67308-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of THP-1 using 67308-1-Ig (same clone as 67308-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    WB analysis of K-562 using 67308-1-Ig (same clone as 67308-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    IHC staining of human liver using 67308-1-Ig (same clone as 67308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    IHC staining of human liver using 67308-1-Ig (same clone as 67308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha 2-Antiplasmin Monoclonal antibody (67308-1-Ig)

    IF Staining of HepG2 using 67308-1-Ig (same clone as 67308-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67308-1-Ig (Alpha-2-antiplasmin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67308-1-PBS in a different storage buffer formulation.

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Alpha 2-Antiplasmin Monoclonal antibody, PBS Only

Alpha 2-Antiplasmin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67308-1-PBS
Clone No.3F6C3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

A2AP, AAP, Alpha 2 antiplasmin, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor, Alpha 2-Antiplasmin, API, PLI, Serpin F2, SERPINF2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67308-1-PBS targets Alpha 2-Antiplasmin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha 2-Antiplasmin fusion protein Ag4095 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Calculated molecular weight 491 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC031592
Gene Symbol Alpha-2-antiplasmin
Gene ID (NCBI) 5345
RRIDAB_2882569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08697
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.