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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Alpha-2-Macroglobulin Monoclonal antibody (66126-1-Ig)

    WB analysis of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Alpha-2-Macroglobulin Monoclonal antibody (66126-1-Ig)

    WB analysis of human brain using 66126-1-Ig (same clone as 66126-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using Alpha-2-Macroglobulin Monoclonal antibody (66126-1-Ig)

    IP experiment of human plasma using 66126-1-Ig (same clone as 66126-1-PBS)

    IP result of anti-Alpha-2-Macroglobulin (IP:66126-1-Ig, 5ug; Detection:66126-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha-2-Macroglobulin Monoclonal antibody (66126-1-Ig)

    IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha-2-Macroglobulin Monoclonal antibody (66126-1-Ig)

    IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha-2-Macroglobulin Monoclonal antibody (66126-1-Ig)

    IF Staining of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (66126-1-Ig, Clone: 4B11F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

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Alpha-2-Macroglobulin Monoclonal antibody, PBS Only

Alpha-2-Macroglobulin Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66126-1-PBS
Clone No.4B11F7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66126-1-PBS targets Alpha-2-Macroglobulin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha-2-Macroglobulin fusion protein Ag19245 相同性解析による交差性が予測される生物種
Full Name alpha-2-macroglobulin
Calculated molecular weight 1474 aa, 163 kDa
Observed molecular weight 185 kDa
GenBank accession numberBC040071
Gene Symbol Alpha 2 Macroglobulin
Gene ID (NCBI) 2
RRIDAB_2881525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01023
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.