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  • Featured Product
  • KD/KO Validated

Alpha Actinin Polyclonal antibody, PBS Only

Alpha Actinin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 11313-2-PBS

Synonyms

Alpha actinin 1, ACTN1, Alpha-actinin cytoskeletal isoform, ACTN 1, actinin, alpha 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha Actinin Polyclonal antibody (11313-2-AP)

    WB analysis using 11313-2-AP (same clone as 11313-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11313-2-AP (Alpha Actinin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Alpha Actinin Polyclonal antibody (11313-2-AP)

    WB analysis of HeLa using 11313-2-AP (same clone as 11313-2-PBS)

    WB result of Alpha Actinin antibody (11313-2-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Actinin transfected HeLa cells. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    WB analysis of mouse spleen using 11313-2-AP (same clone as 11313-2-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    WB analysis of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IHC staining of human colon using 11313-2-AP (same clone as 11313-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11313-2-AP (Alpha Actinin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11313-2-AP (alpha Actinin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66219-1-Ig (N-cadherin) in red. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat heart tissue using Alpha Actinin Polyclonal antibody (11313-2-AP)

    IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using Alpha Actinin Polyclonal antibody (11313-2-AP)

    FC experiment of A431 using 11313-2-AP (same clone as 11313-2-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (11313-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11313-2-PBS targets Alpha Actinin in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Actinin fusion protein Ag1859 相同性解析による交差性が予測される生物種
Full Name actinin, alpha 1
Calculated molecular weight 103 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC015766
Gene symbol Alpha Actinin
Gene ID (NCBI) 87
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.