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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha Antichymotrypsin Monoclonal antibody, PBS Only

Alpha Antichymotrypsin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F2F10

Cat no : 66078-1-PBS

Synonyms

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, Alpha Antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Alpha Antichymotrypsin Monoclonal antibody (66078-1-Ig)

    WB analysis of human plasma using 66078-1-Ig (same clone as 66078-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (Alpha Antichymotrypsin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Alpha Antichymotrypsin Monoclonal antibody (66078-1-Ig)

    WB analysis of human plasma using 66078-1-Ig (same clone as 66078-1-PBS)

    Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. Loading amount:0.7 μL crude plasma was lysised for each lane. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using Alpha Antichymotrypsin Monoclonal antibody (66078-1-Ig)

    WB analysis of human colon using 66078-1-Ig (same clone as 66078-1-PBS)

    Untreated and PNGase F-treated lysates of human colon tissue (25μg/lane) were subjected to SDS PAGE followed by western blot with 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Antichymotrypsin Monoclonal antibody (66078-1-Ig)

    IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Antichymotrypsin Monoclonal antibody (66078-1-Ig)

    IHC staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Antichymotrypsin Monoclonal antibody (66078-1-Ig)

    IHC staining of human liver using 66078-1-Ig (same clone as 66078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Antichymotrypsin Monoclonal antibody (66078-1-Ig)

    IHC staining of human liver using 66078-1-Ig (same clone as 66078-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66078-1-Ig(alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Alpha Antichymotrypsin Monoclonal antibody (66078-1-Ig)

    IF Staining of human tonsillitis using 66078-1-Ig (same clone as 66078-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66078-1-Ig (alpha 1 Antichymotrypsin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66078-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66078-1-PBS targets Alpha Antichymotrypsin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha Antichymotrypsin fusion protein Ag2830 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
Calculated molecular weight 448 aa, 48 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC010530
Gene symbol Alpha 1 Antichymotrypsin
Gene ID (NCBI) 12
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.