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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha B Crystallin Monoclonal antibody, PBS Only

Alpha B Crystallin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4C3

Cat no : 68001-1-PBS

Synonyms

Alpha crystallin B chain, Alpha(B) crystallin, CRYA2, CRYAB, crystallin, alpha B, CTPP2, Heat shock protein beta 5, HSPB5, Rosenthal fiber component

Validation Data Gallery

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  • Western Blot (WB) analysis of rat eye tissue using Alpha B Crystallin Monoclonal antibody (68001-1-Ig)

    WB analysis of rat eye using 68001-1-Ig (same clone as 68001-1-PBS)

    rat eye tissue were subjected to SDS PAGE followed by western blot with 68001-1-Ig (aB-Crystallin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68001-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse eye tissue using Alpha B Crystallin Monoclonal antibody (68001-1-Ig)

    WB analysis of mouse eye using 68001-1-Ig (same clone as 68001-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 68001-1-Ig (aB-Crystallin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using Alpha B Crystallin Monoclonal antibody (68001-1-Ig)

    IHC staining of mouse eye using 68001-1-Ig (same clone as 68001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68001-1-Ig (Alpha B Crystallin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68001-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using Alpha B Crystallin Monoclonal antibody (68001-1-Ig)

    IHC staining of mouse eye using 68001-1-Ig (same clone as 68001-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 68001-1-Ig (Alpha B Crystallin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68001-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68001-1-PBS targets Alpha B Crystallin in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha B Crystallin fusion protein Ag8656 相同性解析による交差性が予測される生物種
Full Name crystallin, alpha B
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight20-22 kDa
GenBank accession numberBC007008
Gene symbol Alpha B Crystallin
Gene ID (NCBI) 1410
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.