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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Alpha E-Catenin Monoclonal antibody (66221-1-Ig)

    WB analysis of NIH/3T3 using 66221-1-Ig (same clone as 66221-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66221-1-Ig (Alpha E catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Alpha E-Catenin Monoclonal antibody (66221-1-Ig)

    IHC staining of human stomach using 66221-1-Ig (same clone as 66221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Alpha E-Catenin Monoclonal antibody (66221-1-Ig)

    IHC staining of human stomach using 66221-1-Ig (same clone as 66221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Alpha E-Catenin Monoclonal antibody (66221-1-Ig)

    IHC staining of human colon using 66221-1-Ig (same clone as 66221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Alpha E-Catenin Monoclonal antibody (66221-1-Ig)

    IHC staining of human colon using 66221-1-Ig (same clone as 66221-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66221-1-Ig (Alpha E catenin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using Alpha E-Catenin Monoclonal antibody (66221-1-Ig)

    FC experiment of MCF-7 using 66221-1-Ig (same clone as 66221-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Alpha E-Catenin (66221-1-Ig, Clone:2B7B8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66221-1-PBS in a different storage buffer formulation.

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Alpha E-Catenin Monoclonal antibody, PBS Only

Alpha E-Catenin Monoclonal Antibody for Indirect ELISA, IHC, WB
Cat No. 66221-1-PBS
Clone No.2B7B8

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

Indirect ELISA, IHC, WB

Synonyms

CTNNA1, Catenin alpha 1, CAP102, Alpha E catenin, alpha catenin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66221-1-PBS targets Alpha E-Catenin in Indirect ELISA, IHC, WB applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Alpha E-Catenin fusion protein Ag23603 相同性解析による交差性が予測される生物種
Full Name catenin (cadherin-associated protein), alpha 1, 102kDa
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC031262
Gene Symbol Alpha E-Catenin
Gene ID (NCBI) 1495
RRIDAB_2881612
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35221
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.