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  • Featured Product
  • KD/KO Validated

Alpha Galactosidase A Monoclonal antibody, PBS Only

Alpha Galactosidase A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B2C5

Cat no : 66121-1-PBS

Synonyms

GLA, GALA, EC:3.2.1.22, Alpha-galactosidase A, Alpha-D-galactoside galactohydrolase

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HEK-293 using 66121-1-Ig (same clone as 66121-1-PBS)

    WB result of GLA antibody (66121-1-Ig, 1:2000) with si-Control and si-GLA transfected HEK293 cells.. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis using 66121-1-Ig (same clone as 66121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of LNCaP using 66121-1-Ig (same clone as 66121-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HeLa using 66121-1-Ig (same clone as 66121-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HEK-293 using 66121-1-Ig (same clone as 66121-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human liver using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human liver using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IF Staining of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IF Staining of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha Galactosidase A antibody (66121-1-Ig, Clone: 2B2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66121-1-PBS targets Alpha Galactosidase A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha Galactosidase A fusion protein Ag7505 相同性解析による交差性が予測される生物種
Full Name galactosidase, alpha
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002689
Gene symbol GLA
Gene ID (NCBI) 2717
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.