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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HEK-293 using 66121-1-Ig (same clone as 66121-1-PBS)

    WB result of GLA antibody (66121-1-Ig, 1:2000) with si-Control and si-GLA transfected HEK293 cells.. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis using 66121-1-Ig (same clone as 66121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HeLa using 66121-1-Ig (same clone as 66121-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HEK-293 using 66121-1-Ig (same clone as 66121-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of LNCaP using 66121-1-Ig (same clone as 66121-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human kidney using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human liver using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human liver using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IF Staining of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IF Staining of HepG2 using 66121-1-Ig (same clone as 66121-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha Galactosidase A antibody (66121-1-Ig, Clone: 2B2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66121-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Alpha Galactosidase A Monoclonal antibody, PBS Only

Alpha Galactosidase A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66121-1-PBS
Clone No.2B2C5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GLA, GALA, EC:3.2.1.22, Alpha-galactosidase A, Alpha-D-galactoside galactohydrolase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66121-1-PBS targets Alpha Galactosidase A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha Galactosidase A fusion protein Ag7505 相同性解析による交差性が予測される生物種
Full Name galactosidase, alpha
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002689
Gene Symbol GLA
Gene ID (NCBI) 2717
RRIDAB_2881520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06280
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.