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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha SNAP Monoclonal antibody, PBS Only

Alpha SNAP Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2A2

Cat no : 67323-1-PBS

Synonyms

Alpha SNAP, NAPA, SNAP alpha, SNAPA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    WB analysis using 67323-1-Ig (same clone as 67323-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    WB analysis using 67323-1-Ig (same clone as 67323-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    IHC staining of mouse cerebellum using 67323-1-Ig (same clone as 67323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    IHC staining of mouse cerebellum using 67323-1-Ig (same clone as 67323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    IF Staining of mouse brain using 67323-1-Ig (same clone as 67323-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    IF Staining of mouse brain using 67323-1-Ig (same clone as 67323-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67323-1-PBS targets Alpha SNAP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha SNAP fusion protein Ag13180 相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor attachment protein, alpha
Calculated molecular weight 33 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC007432
Gene symbol Alpha SNAP
Gene ID (NCBI) 8775
RRIDAB_2882583
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.