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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Alpha SNAP Monoclonal antibody, PBS Only

Alpha SNAP Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E2A2

Cat no : 67323-1-PBS

Synonyms

Alpha SNAP, NAPA, SNAP alpha, SNAPA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    WB analysis using 67323-1-Ig (same clone as 67323-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    WB analysis using 67323-1-Ig (same clone as 67323-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    IHC staining of mouse cerebellum using 67323-1-Ig (same clone as 67323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    IHC staining of mouse cerebellum using 67323-1-Ig (same clone as 67323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    IF Staining of mouse brain using 67323-1-Ig (same clone as 67323-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Alpha SNAP Monoclonal antibody (67323-1-Ig)

    IF Staining of mouse brain using 67323-1-Ig (same clone as 67323-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67323-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67323-1-PBS targets Alpha SNAP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Alpha SNAP fusion protein Ag13180 相同性解析による交差性が予測される生物種
Full Name N-ethylmaleimide-sensitive factor attachment protein, alpha
Calculated molecular weight 33 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC007432
Gene symbol Alpha SNAP
Gene ID (NCBI) 8775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.