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Species Reactivity

Species Reactivity

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Conjugate

Alpha Synuclein Monoclonal antibody, PBS Only (Detector)

Alpha Synuclein Monoclonal Antibody for WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10E9

Cat no : 66412-1-PBS

Synonyms

a-synuclein, SNCA, Non-A4 component of amyloid precursor, Non-A beta component of AD amyloid, NACP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Alpha Synuclein Monoclonal antibody (66412-1-Ig)

    WB analysis of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using Alpha Synuclein Monoclonal antibody (66412-1-Ig)

    WB analysis of rat brain using 66412-1-Ig (same clone as 66412-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50043-1

    Sandwich ELISA standard curve of MP50043-1

    Sandwich ELISA standard curve of MP50043-1, a-Synuclein Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60285-1-PBS. Detection antibody: 66412-1-PBS. Standard: Ag1285. Range: 0.313-20 ng/mL.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Alpha Synuclein Monoclonal antibody (66412-1-Ig)

    IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Alpha Synuclein Monoclonal antibody (66412-1-Ig)

    IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Alpha Synuclein Monoclonal antibody (66412-1-Ig)

    IHC staining of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Alpha Synuclein Monoclonal antibody (66412-1-Ig)

    IHC staining of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Alpha Synuclein Monoclonal antibody (66412-1-Ig)

    IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody (66412-1-Ig, Clone: 1B10E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Alpha Synuclein Monoclonal antibody (66412-1-Ig)

    IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody (66412-1-Ig, Clone: 1B10E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66412-1-PBS targets Alpha Synuclein as part of a matched antibody pair:

MP50043-1: 60285-1-PBS capture and 66412-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Alpha Synuclein fusion protein Ag1285 相同性解析による交差性が予測される生物種
Full Name synuclein, alpha (non A4 component of amyloid precursor)
Calculated molecular weight 14 kDa
Observed molecular weight 18-19 kDa
GenBank accession numberBC013293
Gene symbol Alpha Synuclein
Gene ID (NCBI) 6622
RRIDAB_2881784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.