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Species Reactivity

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  • Featured Product
  • KD/KO Validated

Alpha Tubulin Recombinant antibody, PBS Only (Detector)

Alpha Tubulin Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G7

Cat no : 80762-1-PBS

Synonyms

TUBA1B, Tubulin alpha 1B chain, Tubulin alpha, EC:3.6.5.-, Alpha-tubulin ubiquitous

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alpha Tubulin Recombinant antibody (80762-1-RR)

    WB analysis using 80762-1-RR (same clone as 80762-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80762-1-RR (Tubulin-Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Alpha Tubulin Recombinant antibody (80762-1-RR)

    WB analysis of HEK-293 using 80762-1-RR (same clone as 80762-1-PBS)

    WB result of Alpha Tubulin antibody (80762-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HEK-293 cells. This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Alpha Tubulin Recombinant antibody (80762-1-RR)

    IHC staining of human skin cancer using 80762-1-RR (same clone as 80762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 80762-1-RR (Alpha Tubulin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Alpha Tubulin Recombinant antibody (80762-1-RR)

    IHC staining of mouse brain using 80762-1-RR (same clone as 80762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80762-1-RR (Alpha Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Alpha Tubulin Recombinant antibody (80762-1-RR)

    IHC staining of mouse brain using 80762-1-RR (same clone as 80762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80762-1-RR (Alpha Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using Alpha Tubulin Recombinant antibody (80762-1-RR)

    IF Staining of mouse testis using 80762-1-RR (same clone as 80762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using Alpha Tubulin Recombinant antibody (80762-1-RR)

    IF Staining of C2C12 using 80762-1-RR (same clone as 80762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Alpha Tubulin Recombinant antibody (80762-1-RR)

    IF Staining of HeLa using 80762-1-RR (same clone as 80762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00551-1

    Cytometric bead array standard curve of MP00551-1

    Cytometric bead array standard curve of MP00551-1, Tubulin-Alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80740-1-PBS. Detection antibody: 80762-1-PBS. Standard:Ag18034. Range: 0.098-50 ng/mL.

  • Affinity and Kinetic Characterization of 80762-1-RR

    Affinity and Kinetic Characterization of 80762-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80762-1-RR against Human Alpha Tubulin were performed. The affinity constant is 0.708 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80762-1-PBS targets Alpha Tubulin as part of a matched antibody pair:

MP00551-1: 80740-1-PBS capture and 80762-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag18034 相同性解析による交差性が予測される生物種
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC009314
Gene symbol Alpha Tubulin
Gene ID (NCBI) 10376
RRIDAB_2918911
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.