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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

Alpha Tubulin Recombinant antibody, PBS Only

Alpha Tubulin Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G7

Cat no : 80762-1-PBS

Synonyms

alpha tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, tubulin alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b

Validation Data Gallery

Applications:
  • WB analysis using 80762-1-RR (same clone as 80762-1-PBS)

    WB analysis using 80762-1-RR (same clone as 80762-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80762-1-RR (Tubulin-Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 80762-1-RR (same clone as 80762-1-PBS)

    WB analysis of HEK-293 using 80762-1-RR (same clone as 80762-1-PBS)

    WB result of Alpha Tubulin antibody (80762-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HEK-293 cells. This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 80762-1-RR (same clone as 80762-1-PBS)

    IF Staining of C2C12 using 80762-1-RR (same clone as 80762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 80762-1-RR (same clone as 80762-1-PBS)

    IF Staining of mouse testis using 80762-1-RR (same clone as 80762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80762-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

80762-1-PBS targets Alpha Tubulin in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag18034 相同性解析による交差性が予測される生物種
Full Name tubulin, alpha 1b
Calculated molecular weight 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC009314
Gene symbol TUBA1B
Gene ID (NCBI) 10376
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.