Validation Data Gallery
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Tested Applications
| Positive WB detected in | human skeletal muscle tissue, human heart tissue, pig heart tissue, rat skeletal muscle tissue, pig skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human liver tissue, mouse heart tissue, mouse tongue tissue, rat heart tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue, mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67078-1-Ig targets Alpha Sarcoglycan in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Alpha Sarcoglycan fusion protein Ag28800 相同性解析による交差性が予測される生物種 |
| Full Name | sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) |
| Calculated molecular weight | 387 aa, 43 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC025702 |
| Gene Symbol | alpha Sarcoglycan |
| Gene ID (NCBI) | 6442 |
| RRID | AB_2882386 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha-sarcoglycan is a member of the sarcoglycan-sarcospan complex (SG-SSPN), which is constituted by α/ɛ-, β-, γ-, and δ-SGs as well as SSPN. Alpha-sarcoglycan gene expression is exclusive of striated muscle and is finely regulated during myogenic differentiation, where the amount of alpha-sarcoglycan transcript is selectively increased after differentiation to myotubes (MTs). Alpha sarcoglycan's disruption causes limb-girdle muscular dystrophy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha Sarcoglycan antibody 67078-1-Ig | Download protocol |
| IHC protocol for Alpha Sarcoglycan antibody 67078-1-Ig | Download protocol |
| IF protocol for Alpha Sarcoglycan antibody 67078-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |