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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pancreas tissue using Amylin Polyclonal antibody (22305-1-AP)

    IHC staining of human pancreas using 22305-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22305-1-AP (Amylin Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Amylin Polyclonal antibody (22305-1-AP)

    IHC staining of human pancreas using 22305-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22305-1-AP (Amylin Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using Amylin Polyclonal antibody (22305-1-AP)

    IHC staining of rat pancreas using 22305-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using Amylin Polyclonal antibody (22305-1-AP)

    IHC staining of rat pancreas using 22305-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Amylin Polyclonal antibody (22305-1-AP)

    IHC staining of mouse pancreas using 22305-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Amylin Polyclonal antibody (22305-1-AP)

    IHC staining of mouse pancreas using 22305-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 22305-1-AP (Amylin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Amylin Polyclonal antibody

Amylin Polyclonal Antibody for IHC, ELISA
Cat No. 22305-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, ELISA

Synonyms

Amylin, DAP, Diabetes associated peptide, IAP, IAPP, islet amyloid polypeptide

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22305-1-AP targets Amylin in IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name islet amyloid polypeptide
Calculated molecular weight 10 kDa
GenBank accession numberNM_000415
Gene Symbol Amylin
Gene ID (NCBI) 3375
RRIDAB_2879067
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP10997
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IAPP, also known as Amylin, is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring a tumor of the pancreas that is derived from beta cells and secretes INS. IAPP is co-secreted with INS by pancreatic β-cells and largely contributes to glycemic control. Studies suggest that this protein, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes.

Protocols

Product Specific Protocols
IHC protocol for Amylin antibody 22305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols